Objective: To study the opioid drugs on the rabbit intestinal smooth muscle function and to explore the impact of its possible mechanism. Methods: New Zealand rabbits 56, according to the different experimental methods were randomly divided into groups and in vivo experiments in vitro experimental group. In the experimental group of 17 New Zealand white rabbits were randomly divided into two groups (group A) in 8 cases, the experimental group (group B) 9. Saline were injected through the tube or liquid morphine mixed ink of 5 ml, 30 minutes after the death of acute intestinal measuring the advance of ink in the distance, in the ink of the rate of intestinal advance. In vitro experimental group 28 New Zealand white rabbits were randomly divided into control group (group C), low concentrations of morphine group (Group L), the concentration of morphine group (group M), high concentrations of morphine group (Group H), satisfied Luo increase in the concentration of one morphine group (NM), atropine increase in the concentration of morphine group (AM), and atropine in the concentration of morphine and naloxone (CM group) 4. After the death of rabbits with acute intestinal intestine in vitro perfusion models were designed in accordance with each group different concentrations of drug or a different drug pretreatment temperature constant intestine in vitro perfusion, to be used after reperfusion balance Medlab biological signal acquisition system acquisition in vitro intestine longitudinal and circular muscle tone and frequency curve, the analysis of their implications for the small intestine in vitro intestinal longitudinal and circular muscle tension and contraction of the frequency change. Results: (1) promote the rate of the small intestine: group A, group B was significantly lower intestinal advance, the difference was significant (P <0.05). (2) different concentrations of morphine in vitro intestinal longitudinal and circular muscle tone and frequency of the comparison: Group M, H intestine longitudinal muscle tone was significantly higher than that in group C, the difference was significant (P <0.05), each group changes in the frequency difference was not significant. Group L, M group, Group H intestine circular muscle tone was significantly higher than that in group C (P <0.05), the frequency difference between the groups was not significant. (3) After the effects of different drug concentrations in vitro intestinal morphine on the longitudinal muscle tone and frequency of the comparison: M group, NM group, AM group, CM Group intestine longitudinal muscle tension decreased significantly (P <0.05). ; compared with Group C, CM Group intestine longitudinal muscle tension decreased significantly (P <0.05). AM Group, CM longitudinal muscle group bowel frequency and M and C group were significantly lower (P <0.05). (4) After the effects of different drug concentrations of morphine in vitro intestinal circular muscle tone and frequency of comparison: the M group, NM group, AM group, CM Group intestine circular muscle tension decreased significantly (P <0.05) and Group C, the AM group, CM Group intestine longitudinal muscle tension decreased significantly (P <0.05). AM Group, CM Group intestinal muscle frequency ring and M and C group were significantly lower (P <0.05). Conclusion: opiate morphine can inhibit rabbit in the promotion of bowel movement can be increased in a dose-dependent manner in vitro intestinal rabbits longitudinal and circular muscle tension. In addition to its role in intestinal and bowel opiates and opioid receptor binding regulation after the release of acetylcholine, but also may include other mechanisms for participationSorry,我使用谷歌翻译的~我只是小学生而已,如果能把这个翻译出来,噢,不,如果能把这个读出来,我就去申请吉尼斯世界纪录Objective: To study the opioid drugs on the rabbit intestinal smooth muscle function and to explore the impact of its possible mechanism. Methods: New Zealand rabbits 56, according to the different experimental methods were randomly divided into groups and in vivo experiments in vitro experimental group. In the experimental group of 17 New Zealand white rabbits were randomly divided into two groups (group A) in 8 cases, the experimental group (group B) 9. Saline were injected through the tube or liquid morphine mixed ink of 5 ml, 30 minutes after the death of acute intestinal measuring the advance of ink in the distance, in the ink of the rate of intestinal advance. In vitro experimental group 28 New Zealand white rabbits were randomly divided into control group (group C), low concentrations of morphine group (Group L), the concentration of morphine group (group M), high concentrations of morphine group (Group H), satisfied Luo increase in the concentration of one morphine group (NM), atropine increase in the concentration of morphine group (AM), and atropine in the concentration of morphine and naloxone (CM group) 4. After the death of rabbits with acute intestinal intestine in vitro perfusion models were designed in accordance with each group different concentrations of drug or a different drug pretreatment temperature constant intestine in vitro perfusion, to be used after reperfusion balance Medlab biological signal acquisition system acquisition in vitro intestine longitudinal and circular muscle tone and frequency curve, the analysis of their implications for the small intestine in vitro intestinal longitudinal and circular muscle tension and contraction of the frequency change. Results: (1) promote the rate of the small intestine: group A, group B was significantly lower intestinal advance, the difference was significant (P <0.05). (2) different concentrations of morphine in vitro intestinal longitudinal and circular muscle tone and frequency of the comparison: Group M, H intestine longitudinal muscle tone was significantly higher than that in group C, the difference was significant (P <0.05), each group changes in the frequency difference was not significant. Group L, M group, Group H intestine circular muscle tone was significantly higher than that in group C (P <0.05), the frequency difference between the groups was not significant. (3) After the effects of different drug concentrations in vitro intestinal morphine on the longitudinal muscle tone and frequency of the comparison: M group, NM group, AM group, CM Group intestine longitudinal muscle tension decreased significantly (P <0.05). ; compared with Group C, CM Group intestine longitudinal muscle tension decreased significantly (P <0.05). AM Group, CM longitudinal muscle group bowel frequency and M and C group were significantly lower (P <0.05). (4) After the effects of different drug concentrations of morphine in vitro intestinal circular muscle tone and frequency of comparison: the M group, NM group, AM group, CM Group intestine circular muscle tension decreased significantly (P <0.05) and Group C, the AM group, CM Group intestine longitudinal muscle tension decreased significantly (P <0.05). AM Group, CM Group intestinal muscle frequency ring and M and C group were significantly lower (P <0.05). Conclusion: opiate morphine can inhibit rabbit in the promotion of bowel movement can be increased in a dose-dependent manner in vitro intestinal rabbits longitudinal and circular muscle tension. In addition to its role in intestinal and bowel opiates and opioid receptor binding regulation after the release of acetylcholine, but also may include other mechanisms for participation.
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