|
Experimental only one day to 80 healthy subjects for the goose Heilongjiang seed, in 1,10,30,60. 90 and 120 days old subjects By measuring organ index days to study seed goose with the growth and development of immune organs changes, HE staining and use conventional method of immune organs organizational structure changes with age. SP staining method with the ultra-day growth Vasoactive intestinal peptide (VIP Vasoactive intestinal peptide) in tax organ tissue distribution of qualitative and semi-quantitative analysis of the difference between day and compared. The results showed : thymus, Bursa, Harderian gland and spleen weight increase with age, gradually increasing; Thymus index, Bursa index and Harderian gland, the index increased with age gradually and the spleen index increase with age in the first increased and then decreased. HE Bursa lymphoid follicles were observed with the shape of the day, from day one round into the oval and round. With the shape of the junction of the cortex and medulla of the day, from one day into a circular or irregular-shaped oval. With the days of growth, individual emerged 2-3 lymphoid follicles in the medulla, With the growth of the day and gradually increasing trend of lymphoid follicles. Size and number of lymph follicles in the growth area also increased with age, the number has risen; thymic cortex and medulla of the area is gradually increasing with age, the growth trend But with the growth of age, gradually reducing the proportion of cortical area, a gradual increase in the proportion of medullary area. ～ 60 days old thymic corpuscles growth in the number gradually increased with age, the thymus body size increased with age. area first and then decreased, 60 days maximum. Thymic 10~90 day in the shape of circular, rectangular and irregular shapes, and sizes; 1~90 days with the days of the thickness of the spleen capsule growth is gradually increasing. Periarterial lymphatic sheath in the area for 30 days and 120 days between four changes little. There are 95.0 and 65.3 days in the lymph nodules, but the difference between the number of days each little, Along with the growth of age is gradually increasing the size of the lymph nodules; Harderian gland acinar Rebuilding area and the number increased with age, the growth and the increasing trend, 30, 60, 4 between 90 and 120 days of modest changes, but 30, 60 and 10 days. 90 and 120 days compared to larger changes. Super-staining technique found that 95.0 and 65.3 days in Bursa epithelial spade weak positive reaction found no positive response of epithelial cells submucosal layer inherent in every day are evident intensive VIP-positive nerve fibers in the capsule summary distributed between myometrium point in the day there were fine line kind of VIP-positive nerve fibers and expanded distribution, with the growth of day. positive lymphocytes and immune response of nerve fibers is gradually increasing; The positive reaction to mainly thymic medulla. Cortex days radiate in all weak positive reaction capsule, leaf and vascular connective tissue around 1~90 alive in the days of positive reaction, the reaction was less difference between intensity every day. Medulla this point that day to observe its positive cells lymphocytes found there was an increasing trend, But not increase; in the vascular wall and the spleen capsule 1~90 day was positive reaction 1~90 growth in the days following day, the capsule has weakened the positive trend. 1~90 lymphocytes positive for the red pulp days with the days of growth has increased, but the increase was not; Harderian gland capsule in 1~90 day was positive reaction There were also the days of interstitial lymphocytes and vascular positive, with the growth of day. positive there is a trend of increase in the acinar cells, with 30, 60 and 10 days. 90 and 120 days compared to larger changes; |
